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KMID : 0380019940090010040
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 1 p.40 ~ p.46
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Mathemacical Model for the Production of High-purity Fructo-oligosaccharides by the Mixed-enzyme System of Fructosyltransferase and Glucose Oxidase
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Abstract
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A simplified mathematical model for the production of high-purity fructo-oligosaccharides by the mixed-enzyme system of fructosyl transferase and glucose oxidase was proposed and compared with the experimental results. The kinetic parameters including Km, %*_ and K,; were estimated at 40 `C, in which Km values decreased and K,G and Vma, values increased compared with those of fructosyl transferase alone. The kinetics of the mixed-enzyme system was successfully described in the form of Michaelis-Menten equations. At the reasonable sucrose concentrations tested, the simulated sugar profiles were of good agreement with the experimental ones.
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